Crystal structure of an ascomycete fungal laccase from Thielavia arenaria
نویسندگان
چکیده
منابع مشابه
Biochemical and structural characterisation of the copper containing oxidoreductases catechol oxidase, tyrosinase, and laccase from ascomycete fungi
Catechol oxidase (EC 1.10.3.1), tyrosinase (EC 1.14.18.1), and laccase (EC 1.10.3.2) are copper-containing metalloenzymes. They oxidise substituted phenols and use molecular oxygen as a terminal electron acceptor. Catechol oxidases and tyrosinases catalyse the oxidation of p-substituted o-diphenols to the corresponding o-quinones. Tyrosinases also catalyse the introduction of a hydroxyl group i...
متن کاملCharacterization and heterologous production of a novel laccase from Melanocarpus albomyces
Laccases (EC 1.10.3.2) are multicopper oxidases that catalyze oxidation of various substituted phenolic compounds, aromatic amines and even certain inorganic compounds by using molecular oxygen as the electron acceptor. Their substrate versatility makes laccases highly interesting for various applications, including textile dye bleaching, pulp bleaching and bioremediation, where enzymatic catal...
متن کاملIdentification and characterization of laccase-type multicopper oxidases involved in dye-decolorization by the fungus Leptosphaerulina sp.
BACKGROUND Fungal laccases are multicopper oxidases (MCOs) with high biotechnological potential due to their capability to oxidize a wide range of aromatic contaminants using oxygen from the air. Albeit the numerous laccase-like genes described in ascomycete fungi, ascomycete laccases have been less thoroughly studied than white-rot basidiomycetous laccases. A variety of MCO genes has recently ...
متن کاملExperiences of Kraft Lignin Functionalization by Enzymatic and Chemical Oxidation
Linear hydrophilic derivatives are expected to soften lignin and improve its utilization in composite applications. Oxidation by means of laccase in the presence of oxygen was employed in an attempt to functionalize commercial kraft lignin by vanillic acid-PEG ester and ether derivatives. Thielavia arenaria and Melanocarpus albomyces laccases at pH 6 and 8 were used. According to O2 consumption...
متن کاملReducing the Content of Vocs of Softwood Kraft Lignins for Material Applications
Three laccases, functioning in mild acidic, and one in slightly alkaline conditions, were evaluated in order to reduce low-molecular phenolic VOCs of kraft lignins, which could be used in lignin/natural fibers composites. The potential of a sulfhydryl oxidase to catalyze the oxidation of sulfur containing VOCs (thiols) was also tested in combination with the laccase-catalyzed oxidation. In addi...
متن کامل